Replication of Bacillus subtilis DNA during germination in 5 bromouracil containing medium and marker mapping.

I N the past few years investigations have been made on DNA replication during germination of Bacillus subtiZis spores in $bromouracil (5-BU) containing medium (YOSHIKAWA 1965; OISHI and SUEOKA 1965; and WAKE 1967). Germination of spores in 5-BU containing medium has also been used for mapping of markers by the synchro-transfer method ( OISHI and SUEOKA 1965 and O’SULLIVAN and SUEOKA 1967). In this study we have chosen to examine the relationship between the replication of total DNA and the replication of individual markers during spore germination in 5-BU containing medium. An attempt was made also to map the pur (N) marker by the synchro-transfer method.